CHOC Docs :: Provider Profile

Thomas Kunicki, PhD

Dr. Kunicki has staff privileges at CHOC Orange

Location

Specialty:
Research

Research Focus:
Molecular genetics of thrombosis and haemostasis

Published Works:

Physician Bio

Thomas Kunicki, Ph.D., is a senior scientist with CHOC Childrens’s Research Institute. Dr. Kunicki earned his doctorate at Marquette University. Dr. Kunicki is a regular reviewer for almost 40 scientific journals, including Blood, the New England Journal of Medicine, Nature, British Journal of Hematology, Cell, and Lancet. He is currently founder and chairman of the ThromboGenomics Subcommittee with the International Society of Thrombosis and Hemostasis and a member of the American Society of Hematology.




Research

Dr. Kunicki is dedicated to the research of molecular genetics of thrombosis and haemostasis. He has worked with constant NIH funding since 1978 and in that time, has coauthored more than 100 peer-reviewed publications in journals including Molecular Immunology, Annals of the New York Academy of Science, The Journal of Clinical Investigation, The Journal of Biological Chemistry, Journal of Thrombosis and Haemostasis, Blood Cells and Molecular Disease, and more. Presently, Dr. Kunicki is principal investigator in the NIH-funded study of mouse genes that regulate hemostasis and/or thrombosis.




Published Works

Kunicki TJ, Nugent DJ. 2010. The genetics of normal platelet reactivity. Blood 116:2627- 2634. Epub 2010 Jul 7.

Nurden AT, Kunicki TJ, Nurden P, Fiore M, Martins N, Heilig R, Pillois X. 2010. Mutationanalysis for a patient with Glanzmann thrombasthenia who produced a landmark isoantibody tothe ?IIb?3 integrin. J Thromb Haemost. Epub ahead of print. May 21.

Cheli Y, Williams SA, Ballotti R, Nugent DJ, Kunicki TJ. 2010. Enhanced binding of Poly(ADP-ribose)polymerase-1 and Ku80/70 to the ITGA2 promoter via an extended Cytosine-Adenosine repeat. PLoS ONE. 5:e8743.

Kunicki TJ, Williams SA, Salomon DR, Harrison P, Crisler P, Nakagawa P, Mondala TS, Head SR, Nugent DJ. 2009. Genetics of platelet reactivity in normal, healthy individuals. Journal of Thrombosis and Haemostasis. 7:2116-2122. Epub ahead of print. Sept 9.

Mazzucato M, Cozzi MR, Battiston M, Jandrot-Perrus M, Mongiat M, Marchese P, Kunicki TJ, Ruggeri ZR, De Marco L. 2009. Distinct Spatio-Temporal Ca++ Signaling Elicited by Integrin ?2?1 and Glycoprotein VI under Flow. Blood. 114:2793-2801.

Trifiro E, Williams SA, Cheli Y, Furihata K, Pulcinelli FM, Nugent DJ, Kunicki TJ. 2009. The low frequency isoform of platelet GPVI (GPVIb) attenuates ligand-mediated signal transduction but not receptor expression or ligand binding. Blood 114:1893-1899. Kunicki TJ, Williams SA, Nugent DJ, Harrison P, Segal H, Syed A, Rothwell P. 2009. Lackof association between aspirin responsiveness and seven candidate gene haplotypes in patients with symptomatic vascular disease. Thromb Haemost 101:123-133.

Simon D, Kunicki TJ, Nugent DJ. 2008. Platelet function defects. Haemophilia 14:1240-1249.

Kanaji S, Kanaji T, Migita M, Kunishima S, Kunicki TJ, Okamura T, Izuhara K. 2008. Characterization of a patient with atypical amegakaryocytic thrombocytopenia. European J.Hematol. 80:361-364.

Cheli Y, Jensen D, Marchese P, Habart D, Wiltshire T, Cooke M, Fernandez JA, Ware J, Ruggeri ZM, Kunicki TJ. 2008. The Modifier of hemostasis (Mh) locus on chromosome 4 controls in vivo hemostasis of Gp6-/- mice. Blood 111:1266-1273.

Cheli Y, Kanaji S, Jacquelin B, Chang M, Nugent DJ, and Cheli Y, Kunicki TJ. 2007. Transcriptional and epigenetic regulation of the integrin collagen receptor locus ITGA1-PELOITGA2. Biochimica Biophysica Acta. 1769:546-58..

Cheli Y, Kunicki TJ. 2006. hnRNP L regulates differences in expression of mouse integrin ?2?1. Blood 107(11):4391-8.

Kato K, Furihata K, Cheli Y, Radis-Baptista G, Kunicki TJ. 2006. Effect of multimer size anda natural dimorphism on the binding of convulxin to platelet glycoprotein (GP)VI. Journal of Thrombosis and Haemostasis. 4(5):1107-13.

Inoue O, Suzuki-Inoue K, McCarty OJ, Moroi M, Ruggeri ZM, Kunicki TJ, Ozaki Y, Watson SP. 2006. Laminin stimulates spreading of platelets through integrin alpha-6 beta-1-dependent activation GPVI. Blood, 107:1405-12.

Kunicki TJ, Baronciani L, Canciani MT, Gianniello F, Head SR, Mondala TS, Salomon DR, Federici AB. 2006. An association of candidate gene haplotypes and bleeding severity in von Willebrand Disease (VWD) type 2A, 2B, and 2M pedigrees. Journal of Thrombosis and Haemostasis, 4(1):137-47.

Kunicki TJ, Cheli Y, Moroi M, Furihata K. 2005. The influence of N-linked glycosylation on the function of platelet glycoprotein VI. Blood, 106(8):2744-9.

Michelson AD, Cattaneo M, Eikelboom JW, Gurbel P, Kottke-Marchant K, Kunicki TJ, Pulcinelli FM, Cerletti C, Rao AK. 2005. Aspirin resistance: position paper of the Working Group on aspirin resistance. Journal of Thrombosis and Haemostasis, 3(6):1309-11.

Kanaji S, Kanaji T, Jacquelin B, Chang M, Nugent DJ, Komatsu N, Moroi M, Izuhara K, Kunicki TJ. 2005. Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation. Blood, 105(10):3888-92.

Kunicki TJ, Federici AB, Salomon DR, Koziol J, Head SR, Mondala TS, Chismar JD, Baronciani L, Canciani MT, Peake IR. 2004. An association of candidate gene haplotypes and bleeding severity in Von Willebrand Disease (VWD) type 1 pedigrees. Blood, 104(8):2359-67.

Nurden P, Jandrot-Perrus M, Combrie R, Winckler J, Arocas V, Lecut C, Pasquet JM, Kunicki TJ, Nurden AT. 2004. Severe deficiency of glycoprotein VI in a patient with gray platelet syndrome. Blood, 104(1):107-14.

Vasudevan S, Celikel R, Ruggeri ZM, Varughese KI, Kunicki TJ. 2004. A single amino acid change in the binding pocket alters specificity of an anti-integrin antibody AP7.4 as revealed by its crystal structure. Blood Cells and Molecular Disease, 32(1):176-81.

Kanaji S, Kanaji T, Furihata K, Ware JL, Kunicki TJ. 2003. Convulxin binds to native, human glycoprotein Ib? (GPIb?). J Biol Chem, 278(41):39452-60.

Kato K, Kanaji T, Russell S, Kunicki TJ, Furihata K, Kanaji S, Marchese P, Reininger A, Ruggeri ZM, Ware J. 2003. The contribution of glycoprotein VI to stable platelet adhesion and thrombus formation illustrated by targeted gene deletion. Blood, 102(5):1701-1707.

Jacquelin B, Tuleja E, Kunicki TJ, Nurden P, Nurden AT. 2003. Analysis of platelet membrane glycoprotein polymorphisms in Glanzmann thrombasthenia showed the French gypsy mutation in the alpha IIb gene to be strongly linked to the HPA-1b polymorphism in beta3. J Thromb Haemost., 1(3):573-575.

Furihata K, Kunicki TJ. 2002. Characterization of the human GPVI gene (GP6) 5’-regulatory and promoter regions. Arterioscler Thromb Vasc Biol., 22(10):1733-1739.

Nurden AT, Ruan J, Pasquet J-M, Gauthier B, Combrié R, Kunicki TJ, Nurden P. 2002. A novel 196Leu to Pro substitution in the ?3 subunit of the ?IIb?3 integrin in a patient with a variant form of Glanzmann thrombasthenia. Platelets, 13:101-111.

Furihata K, Clemetson K, Deguchi H, and Kunicki TJ. 2001. Variation in human platelet GPVI content modulates GPVI-specific prothrombinase activity. Arterioscler Thromb Vasc Biol., 21(11):1857-1863.

Jacquelin B, Rozenshteyn D, Kanaji S, Koziol JA, Nurden AT and Kunicki TJ. 2001. Characterization of inherited differences in transcription of the human integrin ?2 gene. J. Biol. Chem., 276(26):23518-235243.

Jacquelin B, Tarantino MD, Kritzik M, Rozenshteyn D, Koziol JA, Nurden AT and Kunicki TJ. 2001. Allele-dependent transcriptional regulation of the human integrin ?2 gene. Blood, 97(6):1721-1726.

Di Paola J, Federici AB, Sacchi E, Canciani MT, Mannucci PM, Kritzik M, Kunicki TJ and Nugent DJ. 1999 Low platelet ?2?1 levels in patients with von Willebrand Disease Type I represent an increased risk factor for bleeding. Blood, 93(11):3578-3582.

Santoso S, Kunicki TJ, Kroll H, Haberbosch W and Gardemann A. 1999. Association of theplatelet glycoprotein Ia C807T gene polymorphism with myocardial infarction in younger patients. Blood, 93(8):2449-2453.

Tomiyama Y, Shiraga M, Kinoshita S, Ambo H, Kurata Y, Matsuzawa Y and Kunicki TJ. 1998. Glanzmann thrombasthenia-like phenotype caused by a defect in inside-out signaling through the integrin ?IIb?3. Thromb. Haemost., 80:735-742.

Kodandapani R, Veerapandian L, Ni C-Z, Chiou C-K, Whital R, Kunicki TJ and Ely KR. 1998. Conformational change in an anti-integrin antibody: Structure of OPG2 Fab bound to a ?3 peptide. Biochem. Biophys. Res. Comm., 251:61-66.

Kritzik M, Savage B, Nugent DJ, Santoso S, Ruggeri ZM and Kunicki TJ. 1998. Nucleotide polymorphisms in the ?2 gene define multiple alleles which are associated with differences in platelet ?2?1. Blood, 92:2382-2388.

Brojer E, Ambo H and Kunicki TJ. 1997. Expression of the murine antibody AP2 specific for the integrin ?IIb?3 complex with the use of bacteriophage lambda vectors. Central-European J. Immunol., 22:225-231.

Kunicki TJ, Annis DS and Felding-Haberman B. 1997. Molecular determinants of Arg-Gly- Asp ligand specificity for ?3 integrins. J.Biol.Chem.,272:4103-4107.

Kunicki TJ, Kritzik M, Annis DS and Nugent DJ. 1997. Hereditary variation in platelet integrin ?2?1 density is associated with two silent polymorphisms in the ?2 gene coding sequence. Blood, 89(6):1939-1943.

Humbert M, Nurden P, Bihour C, Pasquet J-M, Winckler J, Heilmann E, Savi P, Herbert J-M, Kunicki TJ and Nurden AT. 1996. Ultrastructural studies of platelet aggregates from human subjects receiving clopidogrel and from a patient with an inherited defect of an ADP-dependent pathway of platelet activation. Arteriosclerosis, 16:1532-1543.

McKay BS, Annis DS, Honda S, Christie D and Kunicki TJ. 1996. Molecular requirements for assembly and function of a minimized human integrin ?IIb?3. J.Biol.Chem.,271:30544-30547.

Marcinkiewicz C, Rosenthal LA, Mosser DM, Kunicki TJ and Niewarowski S. 1996. Immunological characterization of eristostatin and echistatin binding sites on ?IIb?3 and ? V? 3 integrins. Biochemical Journal, 317:817-825.

Kunicki TJ, Annis DS, Deng YJ, Loftus JC and Shattil SJ. 1996. A molecular basis for affinity modulation of Fab ligand binding to the integrin ?IIb?3 . J.Biol.Chem., 271:20315- 20321.

Humbert M, Kunicki TJ, Bihour C, Winckler J, Nurden AT and Nurden P. 1996. Visualization of activation-dependent epitopes on glycoprotein IIb-IIIa complexes of platelets stimulated by thrombin: Immunogold staining of ultrathin sections. Sem. Thromb. Hemost., 22:279-288.

Nurden P, Humbert M, Piotrowicz RS, Bihour C, Nurden AT and Kunicki TJ. 1996. The distribution of ligand-occupied ?IIb?3 in resting and activated human platelets determined by expression of a novel class of ligand-induced binding site (LIBS) recognized by monoclonal antibody AP6. Blood, 88:887-899.

Schneppenheim R, Brassard J, Krey S, Budde U, Kunicki TJ, Holmberg L, Ware J and Ruggeri ZM. 1996. Defective dimerization of von Willebrand Factor subunits due to a Cys6Arg mutation in Type IID von Willebrand Disease. Pro. Natl. Acad. Sci. U.S.A., 93:3581-3586.

Pelletier AJ, Kunicki TJ and Quaranta V. 1996. Activation of the integrin ?V?3 involves a discrete cation-binding site that regulates conformation. J. Biol. Chem., 271:1364-1370.

Gruel Y, Nugent DJ, Kunicki TJ. 1995. Molecular specificity of anti IIb/IIIa human antibodies. Sem. Thromb. Hemost., 21(1):60-67.

Kunicki TJ, Ely KR, Kunicki T.C., Annis DS. 1995. The exchange of Arg-Gly-Asp (RGD) and Arg-Tyr-Asp (RYD) binding sequences in a recombinant murine Fab fragment specific for the integrin ?IIb?3 does not alter integrin recognition. J. Biol. Chem., 270(28): 16660-16665.

Ely KR, Kunicki TJ, Kodandapani R. 1995 Common molecular scaffold for two unrelated RGD molecules. Protein Engineering, 8:823-827.

Pelletier AJ, Kunicki TJ, Ruggeri ZM, Quaranta V. 1995. The activation state of the integrin ?IIb?3 affects outside-in signals leading to cell spreading and focal adhesion kinase phosphorylation. J.Biol.Chem., 270:18133-18140.

Ishida F, Gruel Y, Brojer E, Nugent DJ, Kunicki TJ. 1995. Repertoire Cloning of a Human IgG Inhibitor of ?IIb?3 Function. The OG Idiotype. Mol. Immunol., 32:613-622.

Kunicki TJ, Honda S, Dawson B, Honda Y, Ruan C and Aster RH. 1995. The PlA1 alloantigen system is a sensitive indicator of the structural integrity of the amino-terminal domain of the human integrin ?3 subunit. Blood Cells, Molecules and Diseases. 21:131-141.

Honda S, Honda Y, Bauer B, Ruan C and Kunicki TJ. 1995. The impact of threedimensional structure on the expression of PlA alloantigens on human integrin ?3. Blood, 86:234-242.

Honda S, Tomiyama Y, Pelletier AJ, Annis DS, Honda Y, Orchekowski R, Ruggeri Z and Kunicki TJ. 1995. Topography of ligand-induced binding sites (LIBS), including a novel cation-sensitive epitope (AP5) at the amino-terminus, of the human integrin ?3 subunit. J.Biol.Chem., 270:11947-11954.

Kodandapani R, Veerapandian B, Kunicki TJ, Ely KR. 1995. Crystal structure of the OP-G2 Fab: An anti-receptor antibody that mimics an RGD cell adhesion site. In J. Biol. Chem., 270:2268-2273.

Gruel Y, Brojer E, Nugent DJ, Kunicki TJ. 1994. Further characterization of the thrombasthenia-related idiotype OG. Anti-idiotype binds to an epitope(s) shared by the fibrinogen B? chain, vitronectin and von Willebrand Factor. J. Exp. Med., 180:2259-2267.

Kouns WC, Steiner B, Kunicki TJ, Moog S, Jutzi J, Jennings LK, Lanza F. 1994. Reformation of the fibrinogen binding site on platelets isolated from a patient with the Strasbourg I variant of Glanzmann thrombasthenia. Blood, 84:1108-1115.

Tarantino M, Kunicki TJ, Nugent DJ. 1994. The estrogen receptor is present in human megakaryocytes. Ann.N.Y. Acad.Sci. 714:293-296.

Cadroy Y, Hanson SR, Kelly AB, Marzec UM, Evatt BL, Kunicki TJ, Montgomery RR, Harker LA. 1994. Relative antithrombotic effects of monoclonal antibodies targeting different platelet glycoprotein-adhesive molecule interactions in nonhuman primates. Blood 83:3218- 3224.

Kunicki TJ, Orchekowski R, Annis DS, Honda Y. 1993. Variability of integrin ?2?1 activity on human platelets. Blood 82:2693-2703.

Celikel R, Williamson MM, Ely KR, Kunicki TJ. 1993. Crystallization of the OP-G2 Fab fragment: a fibrinogen mimic with specificity for the platelet glycoprotein IIb-IIIa. Acta Crystallographica D49:421-422.

Kunicki TJ, PJ Newman. 1992. The molecular immunology of human platelet proteins. Blood. 80:1386-1404.

Tomiyama Y, Kunicki TJ, Zipff T, Ford SB, Aster RH. 1992. Response of human platelets toactivating monoclonal antibodies: importance of Fc?RII (CD32) phenotype. Blood 80:2261-2268.

Chen YQ, Gao G, Timar T, Tang D, IM Grossi, M Chelladurai, Kunicki TJ, SEG Fligie, JD Taylor, KV Honn. 1992. Identification of the ?IIb? 3 integrin in murine tumor cells. J Biol Chem. 267:17314-17320.

Jallu V, M Pico, J Laroche, J Chevaleyre, G Vezon, Kunicki TJ, AT Nurden. 1992. Characterization of an antibody to the integrin ?3 subunit (GPIIIa) in the plasma of a patient with Glanzmann's thrombasthenia whose first child was born with neonatal thrombocytopenia. Human Antibodies and Hybridomas. 3:93-106.

Tomiyama Y, E Brojer, Z Ruggeri, T Kieber-Emmons, SJ Shattil, J Smiltneck, J Gorski, A Kumar, TJ Kunicki. 1992. A molecular model of RGD ligands: Antibody D gene segments that direct specificity for the integrin ?IIb?3. J Biol Chem. 267:18085-18092.

Tomiyama Y, T Tsubakio, RS Piotrowicz, Y Kurata, JC Loftus, TJ Kunicki. 1992. The Arg-Gly-Asp (RGD) recognition site of platelet glycoprotein IIb-IIIa on nonactivated platelets is accessible to high-affinity macromolecules. Blood 79:2303-2312.

Tomiyama Y, R Kekomaki, J McFarland, TJ Kunicki. 1992. Anti-vinculin antibodies in sera of patients with immune thrombocytopenia and normal subjects. Blood 79:161-168.

Primm JG and TJ Kunicki. 1992. Inexpensive manual synthesis of anti-thrombotic peptides. Nouv Rev Fr Hematol 34:143-147.

Kunicki TJ, DS Annis, J Gorski and DJ Nugent. 1991. Nucleotide sequence of the human autoantibody 2E7 specific for the platelet integrin IIb heavy chain. J. Autoimmunity 4:433-446.

Wencel-Drake J, DS Annis, JR Okita, TJ Kunicki. 1991. Activation of calpain I and hydrolysis of calpain substrates (ABP, GPIb and talin) are not a function of thrombin-induced platelet aggregation. Arteriosclerosis and Thrombosis 11:882-891.

Kekomaki R, B Dawson, J McFarland and TJ Kunicki. 1991. Localization of human platelet autoantigens to the cysteine-rich region of glycoprotein IIIa. J. Clin. Invest. 88:847-854.

Kunicki TJ, EF Plow, R Kekomaki and DJ Nugent. 1991. Human monoclonal auto-antibody 2E7 is specific for a peptide sequence of platelet glycoprotein IIb. Localization of the epitope to IIb231-238 with an immunodominant Trp235. J. Autoimmunity 4:415-431.

Kunicki TJ, K Furihata, R Kekomaki, JP Scott and DJ Nugent. 1990. A human monoclonal autoantibody specific for human platelet glycoprotein IIb (integrin ?IIb) heavy chain. Human Antibodies and Hybridomas 1:83-95.

Smith JW, ZM Ruggeri, TJ Kunicki and DA Cheresh. 1990. Interaction of integrins ?v?3 and glycoprotein IIb-IIIa with fibrinogen. Differential peptide recognition accounts for distinct binding sites. J. Biol. Chem. 265:12267-12271.

Miller JL, KO Hustad, JM Kupinski, VA Lyle and TJ Kunicki. 1990. Increased platelet sensitivity to ristocetin is predicted by the binding characteristics of a GPIb/IX determinant. Br. J. Haematol. 74:313-319.

Okita JR, MM Frojmovic, S Kristopeit, T Wong and TJ Kunicki. 1988. Montreal Platelet Syndrome: Decreased activity of calcium-activated neutral proteinases (calpains). Blood 74:715-721.

Simon TL, J Collins, TJ Kunicki, K Furihata, KJ Smith and RH Aster. 1988.Post-transfusion purpura associated with alloantibody specific for the platelet antigen, Pena. Am. J. Hematology. 29:38-40.

Stevens MCG, VS Blanchette, MH Freedman, C Sparling and TJ Kunicki. 1988. A variant form of Bernard-Soulier syndrome: Minimal haemostatic defect associated with partial platelet GPIb deficiency. Clin. and Lab. Haematol. 10:443-451.

Wayner EA, WG Carter, RS Piotrowicz and TJ Kunicki. 1988. The function of extracellular matrix receptors in mediating cell adhesion to extracellular matrix: Preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa. J. Cell Biol. 107:1881-1891.

Kickler TS, JH Herman, K Furihata, TJ Kunicki and RH Aster. 1988. Identification of Bakb, a new platelet-specific antigen associated with post-transfusion purpura. Blood 71:894-898.

Kunicki TJ, DJ Nugent, EA Wayner and WG Carter. 1988. The human fibroblast class II extracellular matrix receptor (ECMR II) mediates platelet adhesion to collagen and is identical to the platelet glycoprotein Ia-IIa complex. J. Biol. Chem. 263:4516-4519.

Piotrowicz RS, RP Orchekowski, DJ Nugent, KM Yamada and TJ Kunicki. 1988. Glycoprotein Ic-IIa functions as an activation-independent fibronectin receptor of human platelets. J. Cell Biol.106:1359-1364

Furihata K, J Hunter, RH Aster, GR Koewing, NR Shulman and TJ Kunicki. 1988. Human anti-PlE1 antibody recognizes epitopes associated with the alpha subunit of platelet glycoprotein Ib. Br. J. Haematol. 68:103-110.

Hanson SR, FI Pareti, ZM Ruggeri, TJ Kunicki, RR Montgomery, TS Zimmerman and LA Harker. 1988. Effects of a monoclonal antibody (AP2) against the platelet glycoprotein IIb-IIIa complex on thrombosis and hemostasis in the baboon. J. Clin. Invest. 81:149-158.

Furihata K, DJ Nugent, A Bissonette, RH Aster and TJ Kunicki. 1987. On the association of the platelet-specific alloantigen, Pen, with glycoprotein IIIa. Evidence for heterogeneity ofglycoprotein IIIa. J. Clin. Invest. 80:1624-1630.

Nugent DJ, TJ Kunicki, C Berglund and ID Bernstein. 1987. A human monoclonal autoantibody recognizes a neoantigen on glycoprotein IIIa expressed on stored and activated platelets. Blood 70:16-22.

Gottschall JL, J Collins, TJ Kunicki, R Nash and RH Aster. 1987. Effect of hemolysis on apparent values of platelet-associated IgG. Am. J. Clin. Pathol. 87:218-222.

Newman PJ, RP McEver, MP Doers and TJ Kunicki. 1987. Synergistic action of two murine monoclonal antibodies that inhibit ADP-induced platelet aggregation without blocking fibrinogen binding. Blood 69:668-676.

Lai C-S, M Wirt, J-J Yin, W Froncisz, JB Feix, TJ Kunicki and JS Hyde. 1986. Lateral diffusion of lipid probes in the surface membrane of human platelets. An electron- electron double resonance (ELDOR) study. Biophys. J. 50:503-506.

Ginsberg MH, A Lightsey, TJ Kunicki, A Kaufman, G Marguerie and EF Plow. 1986. Divalent cation regulation of the surface orientation of platelet membrane glycoprotein IIb: Correlation with fibrinogen binding function and definition of a novel variant of Glanzmann's thrombasthenia. J. Clin. Invest. 78:1103-1111.

Kunicki TJ, DJ Nugent, RS Piotrowicz and C-S Lai. 1986. Covalent attachment of sulfhydryl-specific, electron spin resonance (ESR) labels to Fab' fragments of murine monoclonal antibodies that recognize human platelet mem- brane glycoproteins. Development of membrane protein-specific spin probes. Biochemistry 25:4979-4983.

Wencel-Drake JD, EF Plow, TJ Kunicki, V Woods, D Keller and MH Ginsberg. 1986. Localization of internal pools of membrane glycoproteins involved in platelet adhesive response. Am. J. Pathol. 124:324-334.

George JN, EB Pickett, S. Saucerman, RP McEver, TJ Kunicki, N Kieffer and PJ Newman.1986. Platelet surface glycoproteins: studies on resting and activated platelets and plateletmembrane microparticles in normal subjects, and observations in patients during adultrespiratory distress syndrome, cardiac surgery, and hemodialysis. J. Clin. Invest., 78:340-348.

Newman PJ, Y Kawai, RR Montgomery and TJ Kunicki. 1986. Synthesis by cultured human umbilical vein endothelial cells of two proteins structurally and immunologically related to platelet membrane glycoproteins IIb and IIIa. J. Cell Biol., 103:81-86.

Patterson WR, TJ Kunicki and TG Bell. 1986. Two-dimensional electrophoretic studies of platelets from dogs affected with Basset Hound hereditary thrombopathy: A thrombasthenia-like aggregation defect. Thromb. Res. 42:195-203.

Gill JC, P Carlson, TJ Kunicki and RH Aster. 1986. A naturally occurring, warm- reactive macroglobulin specific for papain-activated human platelets: Preliminary characterization. Am. J. Hematol. 21:189-199.

Pidard D, D Didry, TJ Kunicki and AT Nurden. 1986. Temperature-dependent effects of EDTA on the membrane glycoprotein IIb-IIIa complex and platelet aggregability. Blood 67:604-611.

Szatkowski NS, TJ Kunicki and RH Aster. 1986. Identification of glycoprotein Ib as a target for autoantibody in idiopathic (autoimmune) thrombocytopenic purpura. Blood 67:310-315.

Lombardo VT, E Hodson, J Roberts, TJ Kunicki, TS Zimmerman and ZM Ruggeri. 1985. Independent modulation of von Willebrand factor and fibrinogen binding to the platelet membrane glycoprotein IIb/IIIa complex as demonstrated by monoclonal antibody. J. Clin. Invest. 76:1950-1958.

Kunicki TJ and PJ Newman. 1985. Synthesis of analogs of human platelet membrane glycoprotein IIb-IIIa by chicken peripheral blood thrombocytes. Pro. Natl. Acad. Sci. U.S.A. 82:7319-7323.

Kunicki TJ, PJ Newman, DL Amrani and MW Mosesson. 1985. Human platelet fibrinogen: Purification and hemostatic properties. Blood 66:808-815.

Brass LF, SJ Shattil, TJ Kunicki and JS Bennett. 1985. Effect of calcium on the stability of the platelet membrane glycoprotein IIb-IIIa complex. J. Biol. Chem. 260:7875-7881.

Okita JR, D Pidard, PJ Newman, RR Montgomery and TJ Kunicki. 1985. On the association of glycoprotein Ib and actin-binding protein in human platelets. J. Cell Biology 100:317-321.

Newman PJ, RW Allen, RA Kahn and TJ Kunicki. 1985. Quantitation of membrane glycoprotein IIIa on intact human platelets using the monoclonal antibody, AP-3. Blood 65:227-232.

Kunicki TJ, RR Montgomery and J Schullek. 1985. Cleavage of human von Willebrand factor by platelet calcium-activated protease. Blood 65: 352-356.

Rosa J-P, N Kieffer, D Didry, D Pidard, TJ Kunicki and AT Nurden. 1984. The human platelet membrane glycoprotein complex GP IIb-IIIa expresses antigenic sites not exposed on the dissociated glycoproteins. Blood 64:1246-1253.

Kristopeit SM and TJ Kunicki. 1984. Quantitation of platelet membrane glycoproteins in Glanzmann's thrombasthenia and the Bernard-Soulier syndrome by electroimmunoassay. Thrombosis Res. 36:133-142.

Kunicki TJ, MW Mosesson and D Pidard. 1984. Cleavage of fibrinogen by human platelet calcium-activated protease. Thrombosis Res. 35:169-182.

Bellone JD, TJ Kunicki and RH Aster. 1983. Immune thrombocytopenia associated with carcinoma. Ann. Int. Med. 99:470-472.

Pidard D, RR Montgomery, JS Bennett and TJ Kunicki. 1983. Interaction of AP-2, a monoclonal antibody specific for the human platelet glycoprotein IIb-IIIa complex, with intact platelets. J. Biol. Chem. 258:12582-12586.

Montgomery RR, TJ Kunicki, C Taves, D Pidard and M Corcoran. 1983. The diagnosis of Bernard-Soulier syndrome and Glanzmann's thrombasthenia with a monoclonal assay on whole blood. J. Clin. Invest. 71:385-389.

Nurden AT, D Dupuis, D Pidard, N Kieffer, TJ Kunicki and J-P Cartron. 1982. Surface modifications in the platelets of a patient with ?-N-Acetyl-D-Galactosamine residues, the Tn-Syndrome. J. Clin. Invest. 70:1281-1291

Pidard D, J-P Rosa, TJ Kunicki and AT Nurden. 1982. Further studies on the interaction between human platelet membrane glycoproteins IIb and IIIa in Triton X-100. Blood 60:894-904.

Nurden AT, TJ Kunicki, D Dupuis, C Soria and JP Caen. 1982. Specific protein and glycoprotein deficiencies in platelets isolated from two patients with the Gray platelet syndrome. Blood 59:709-718.

Kunicki TJ, MB Koenig, SM Kristopeit and RH Aster. 1982. Direct quantitation of total platelet-associated IgG by electroimmunoassay. Blood 60:54-58.

Kunicki TJ, AT Nurden, D Pidard, NR Russell and JP Caen. 1981. Characterization of human platelet glycoprotein antigens giving rise to individual immunoprecipitates in crossed immunoelectrophoresis. Blood 58:1190-1197.

Caen JP, AT Nurden and TJ Kunicki. 1981. Inherited abnormalities of platelet glycoproteins. Phil. Trans. R. Soc. Lond. B 294:281-290.

Nurden AT, D Dupuis, D Pidard, TJ Kunicki and JP Caen. 1981. Biochemistry and immunology of platelet membranes with reference to glycoprotein composition. Annals N.Y. Acad. Sci. 370:72-86.

Kunicki TJ, D Pidard, J-P Rosa and AT Nurden. 1981. The formation of Ca++- dependent complexes of platelet membrane glycoproteins IIb and IIIa in solution as determined by crossed immunoelectrophoresis. Blood 58:268-278.

Kunicki TJ, DJ Christie, JL Winkelhake and RH Aster. 1981. Alcian blue as a protein stain for sodium alkyl sulfate-polyacrylamide gels: Application to analysis of polypeptide components of the human platelet. Anal. Biochem. 110:412-419.

Nurden AT, D Dupuis, TJ Kunicki and J Caen. 1981. Analysis of the glycoprotein and protein composition of Bernard-Soulier platelets in single and two-dimensional SDS polyacrylamide gel electrophoresis. J. Clin. Invest. 67:1431-1440.

Kunicki TJ, N Russel, AT Nurden, RH Aster and JP Caen. 1981. Further studies of the human platelet receptor for quinine-and quinidine-dependent antibodies. J. Immunol. 126:398-402.

Kunicki TJ, D Pidard, J-P Cazenave, AT Nurden and JP Caen. 1981. Inheritance of the human platelet alloantigen, PlA1, in Type I Glanzmann's thrombasthenia. J. Clin. Invest. 67:717-724.

Winkelhake JL, TJ Kunicki, BM Elcombe, RJ Chang and RH Aster. 1980. Effect of pH treatments and deglycosylation of rabbit IgG on binding of C1q. J. Biol. Chem. 255:2822-2828.

Hamid MA, TJ Kunicki and RH Aster. 1980. Lipid composition of freshly prepared and stored platelet concentrates. Blood 55:124-130.

Nurden AT, I Hagen, TJ Kunicki and JP Caen. 1979. Etude immunochimique des anomalies plaquettes de sujets atteints de thrombasthenie de Glanzmann et de syndrome de Bernard-Soulier. Comptes Rendus 289:209-211.

Kunicki TJ and RH Aster. 1979. Isolation and immunologic characterization of the human platelet alloantigen, PlA1. Molecular Immunology 16:353-360.

Kunicki TJ, MM Johnson and RH Aster. 1978. Absence of the receptor for drug- dependent antibodies in platelets of patients with Bernard-Soulier syndrome. J. Clin. Invest. 62:716-719.

Kunicki TJ and RH Aster. 1978. Deletion of the platelet-specific alloantigen PlA1 from platelets in Glanzmann's thrombasthenia. J. Clin. Invest. 61:1225-1231.

Kunicki TJ, M Tucelli, GA Becker and RH Aster. 1975. A study of variables affecting the quality of platelets stored at room temperature. Transfusion 15:414-421.

Becker GA, TJ Kunicki and RH Aster. 1974. Effect of prostaglandin E-1 on harvesting of platelets from refrigerated whole blood. J. Lab. Clin. Med. 83:304-309.

Becker GA, M Tucelli, TJ Kunicki, MK Chalos and RH Aster. 1973. Studies of platelet concentrates stored at 22oC and 4oC. Transfusion 13:61-68.

Invited Reviews, Editorials and Book Chapters
Kunicki TJ. 2009. A Mischief of Mice. Blood. 114:5249-5250.

Kunicki TJ. 2009. CLEC ..too! Blood. 114:3364-3365.

Kunicki TJ and Nugent DJ. 2009. Chapter 56: Qualitative Platelet Disorders. In: Wintrobe’s Clinical Hematology (12th Edition). Eds. JP Greer, J Foerster, JN Lukens. Brandon/Hill, Nashville, TN. pp 1361-1378.

Kunicki TJ and Nugent DJ. 2006. Human Platelet Antigens. In: Blood Banking and Transfusion Medicine. (Second Edition). Eds. CD Hillyer, LE Silberstein, PM Ness and KN Anderson. W.B. Saunders Company, Philadelphia, PA. pp.63-80.

Kunicki TJ. 2006. Platelet Immunology. In: Hemostasis and Thrombosis: Basic Principles and Clinical Practice (Fifth Edition). Eds. RN Colman, VJ Marder, AW Clowes, JN George, SZ Goldhaber. Lippincott Williams & Wilkins. Philadelphia, PA. Chapter 25, pp. 461- 478.

Kunicki TJ and Nugent DJ. 2005. Human platelet antigens. In: Hematology: Basic Principles and Practice (Fourth Edition). Eds. R Hoffman, EJ Benz, Jr., SJ Shattil, B Furie, HJ Cohen, LE Silberstein and P McGlave. Churchill Livingston, New York, NY.

Kunicki TJ, Head S, Salomon DR. 2004. Platelet receptor structures and polymorphisms. In: Methods in Molecular Biology Volume 273, Platelets and Megakaryocytes, Volumen 2 Perspective and Techniques. Eds. J.M. Gibbins and M.P. Mahaut-Smith. Humana Press. Totowa, MJ. Chapter 28, pp. 455-478.

Nugent DJ and Kunicki TJ. 2004. Immune-Mediated Hematologic and Oncologic Disorders. In:Immunologic Disorders in Infants and Children, 5th Edition. Eds. E.R. Stiehm, H.D. Ochs, and J.A. Winkelstein. Elsevier Sanders. Philadelphia, PA. Chapter 39, pp. 1253-1282.

Kunicki TJ and Nugent DJ. 2003. Human Platelet Antigens. In: Blood Banking and Transfusion Medicine. Eds. CD Hillyer, LE Silberstein, PM Ness and KN Anderson. W.B. Saunders Company, Philadelphia, PA. pp.63-80

Kunicki TJ and Nugent DJ. 2002. The influence of platelet glycoprotein polymorphisms on receptor function and risk for thrombosis. In: Vox Sang. 83, (Suppl. 1):085-090.

Jacquelin B, Nurden AT, and Kunicki TJ. 2002. What are the platelet receptors for collagen? In: Sang Thrombose Vaisseaux (French). May; 14(5):291-298.

Kunicki TJ. 2002. Gene regulation of platelet function. In: Platelets in Thrombotic and non- Thrombotic Disorders: Pathophysiology, Pharmacology and Therapeutics. Eds. P. Gresele, CP Page, V. Fuster and J Vermylen. Cambridge University Press, Cambridge, UK. pp. 760-779.

Furihata K, Nugent DJ, and Kunicki TJ. 2002. Influence of platelet collagen receptor polymorphisms on risk for arterial thrombosis. In: Archives of Pathology & Laboratory Medicine. Mar;126(3):305-309.

Kunicki TJ. 2002. The Influence of Platelet Collagen Receptor (Integrin ?2?1 and GPVI) Polymorphisms in Hemostasis and Thrombotic Disease. In:Arteriosclerosis, Thrombosis, and Vascular Biology. 22:14-20.

Kunicki TJ. 2001. Platelet Immunology. In: Hemostasis and Thrombosis: Basic Principles and Clinical Practice (Fourth Edition). Eds. RW Colman, J Hirsh, VJ Marder, AW Clowes, JN George. Lippincott Williams & Wilkins. Philadelphia, PA. pp. 461-478.

Santoso S and Kunicki TJ. 2001. Platelet receptor polymorphisms and thrombotic risk. In: Italian Heart Journal. Ed. A. Maseri. Nov;(2):811-815.

Kunicki TJ. 2001. Platelet integrins and other adhesion receptors – An update. Risk prediction in stroke and coronary artery disease. In: European Thrombosis Research Organization (ETRO), Thrombophilia and Artherothrombosis, Scientific and Educational Symposium. pp. 22-24.

Kunicki TJ and Ruggeri ZM. 2001. Platelet Collagen Receptors and Risk Prediction in Stroke and Coronary Artery Disease. In:Circulation. 104:1451-1453.

Kunicki TJ. 2001. The Role of Platelet Collagen Receptor (Glycoprotein la/lla; Integrin ?2?1) Polymorphisms in Thrombotic Disease. In:Current Opinion in Hematology. Ed. T. Stossel. Lippincott Williams & Wilkins, Philadelphia, PA. pp. 277-285.

Kunicki TJ. 2000. Human platelet antigens. In: Hematology: Basic Principles and Practice (Third Edition). Eds. R Hoffman, EJ Benz, Jr., SJ Shattil, B Furie, HJ Cohen, LE Silberstein and P McGlave. Churchill Livingston, New York, NY. Pp. 2206-2208.

Bussel JB, Kunicki TJ, Michelson AD. 2000. Platelets: New Understanding of Platelet Glycoproteins and their role in disease. In: Hematology: American Society of Hematology Education Program Book. American Society of Hematology, Washington, DC. pp. 222-240.

Kunicki TJ. 1998. Molecular requirements for ligand recognition by ?3 integrins. In: Proceedings of the XIth International Symposium on Atherosclerosis. Eds. B Jacotot, D Mathé, JC Fruchart. Elsevier, Amsterdam. pp. 1119-1125.

Kunicki TJ, Savage B. 1998. The involvement of integrins ?IIb?3 and ? 2?1 in von Willebrand Factor-mediated platelet functions. In: von Willebrand Factor. Ed. Z Ruggeri. R.G. Landes Co., Austin, TX. pp. 141-185.

Kunicki TJ, Nugent DJ. 1997. Idiotypes of Anti-Platelet Antibodies. In: Idiotypes in Medicine: Autoimmunity, Cancer and Infectious Diseases. Eds. S Ferone, R Kennedy, Y Shoenfeld. Elsevier, pp. 165-187.

Kunicki TJ. 1995. The role of platelets in hemostasis. In: Principles of Transfusion Medicine. Eds. EC Rossi, T Simon, G Moss. Williams and Wilkins, New York. 2nd Edition, pp. 221- 230.

Honda S, Felding-Haberman B, Loftus J, Annis DS, Kunicki TJ. 1995. Report of the CD41, CD61 subsection of the platelet section: Localization of epitopes on integrins ?IIb?3 (CD41/CD61) and ? V? 3 (CD51/CD61). In: Leucocyte Typing V: White Cell Differentiation Antigens. Eds. SF Schlossman, L Boumsell, W Gilks, JM Harlan, T Kishimoto, C Morimoto, J Ritz, Silverstein RL, Springer TA, Tedder TF and Todd RF. Oxford University Press, Oxford, England. Vol. 2, pp. 1293-1298.

Kunicki TJ. 1994. Human platelet antigens. In: Hematology: Basic Principles and Practice (Second Edition). Eds. EJ Benz, HJ Cohen, B Furie, R Hoffman, SJ Shattil. Churchill Livingstone, New York, pp. 1961-1974.

Kunicki TJ, ZR Ruggeri. 1994. Molecular properties of human platelet integrin ?IIb?3. In: Integrins: Molecular and Biological Responses to the Extracellular Matrix. Eds. Mecham RP and Cheresh D. Academic Press, Inc., San Diego, pp. 195-235.

Kunicki TJ. 1992. Human platelet antigens. In: Hematology: Basic Principles and Practice. Eds. EJ Benz, HJ Cohen, B Furie, R Hoffman, SJ Shattil. Churchill Livingstone, New York, pp. 1556-1565.

Kunicki TJ. 1992. The role of platelets in hemostasis. In: Principles of Transfusion Medicine. Eds. EC Rossi, T Simon, G Moss. Williams and Wilkins, New York, pp 181-192.

Kunicki TJ. 1990. The Molecular Biology of Platelet Glycoproteins. Transfusion Medicine Reviews. 4:91-97.

Kunicki TJ. 1989. Platelet membrane glycoproteins and their function: An overview. Blut 59:30-34.

Aster RH and TJ Kunicki. 1989. Platelet immunology: historical perspective and future directions. In: The Immunobiology of Platelets: Molecular and Clinical Aspects. Eds. TJ Kunicki and JN George. J.B. Lippincott, Philadelphia, pp. 3-5.

Kunicki TJ. 1989. Biochemistry of platelet-associated isoantigens and alloantigens. In: The Immunobiology of Platelets: Molecular and Clinical Aspects. Eds. TJ Kunicki and JN George. J.B. Lippincott, Philadelphia, pp. 99-120.

Kunicki TJ and DJ Beardsley. 1988. The alloimmune thrombocytopenias: neonatal alloimmune thrombocytopenic purpura and post-transfusion purpura. In: Progress in Hemostasis and Thrombosis, Volume 9. Ed. B.S. Coller. Grune and Stratton, New York. InPress.

Kunicki TJ. 1988. Platelet glycoprotein antigens and immune receptors. In: Receptor Mechanisms in Platelet Function. Progress in Clinical and Biological Research. Alan R. Liss, Inc., New York. Ed. GA Jamieson, pp. 85-121.

Kunicki TJ. 1988. Human platelet antigen systems. In: Platelets. Eds. DM Smith and SH Summers. American Association of Blood Banks, Arlington, VA, pp. 15-53.

Kunicki TJ, K Furihata and D Nugent. 1988. Glycoprotein IIb-IIIa as an immunologic target. In: Platelet Membrane in Transfusion Medicine. Eds. F. Decary and G. Rock. S. Karger, Basel, pp. 44-63.

Kunicki TJ, K Furihata, B Bull and D Nugent. 1987. The immunogenicity of platelet membrane glycoproteins. Transfusion Medicine Reviews 1:21-33.

Kunicki TJ, PJ Newman, RR Montgomery, Y Kawai and K Furihata. 1987. The human platelet membrane glycoprotein IIb-IIIa complex: A prototype for cytoadhesins. In: Biology and Pathology of Platelet-Vessel Wall Interactions. Eds., G. Jolles, Y. LeGrand, A.T. Nurden. Academic Press, Inc., London, pp. 201-218.

Kunicki TJ, RR Montgomery and SM Kristopeit. 1986. Polyclonal and monoclonal antibodies used in the rapid detection of platelet defects. In: Monoclonal Antibodies and Human Blood Platelets. Ed. J. McGregor. Elsevier Science Publishers, Amsterdam, pp. 297-305.

Kunicki TJ and PJ Newman. 1986. The biochemistry of platelet-specific alloantigens. In: Platelet Serology: Research Progress and Clinical Implications. Eds. F. DeCary and G. Rock. S. Karger, Basel, pp. 18-32.

Montgomery RR, TJ Kunicki and LM Glode. 1986. Use of monoclonal antibody to increase the sensitivity and specificity of precipitating immunoassays and cell surface binding immunoassays. Methods in Enzymology 121:702-717.

Kunicki TJ. 1985. "Organization of glycoproteins within the platelet plasma membrane." In: Platelet Membrane Glycoproteins. Eds., J.N. George, A.T. Nurden, D.R. Phillips. Plenum Press, New York. pp. 87-103.

Kunicki TJ. 1983. "Platelet antigens." In: Non-HLA Antigens in Health, Aging and Malignancy. Eds., E. Cohen and D.P. Singal. Alan R. Liss Publications, New York, pp 31-46.

Kunicki TJ, DJ Christie and RH Aster. 1983. The human platelet receptor(s) for quinine/quinidine-dependent antibodies. Blood Cells 9:293-301.

Kunicki TJ and RH Aster. 1983. "Qualitative and quantitative tests for platelet alloantibodies and drug-dependent antibodies." In: Methods in Hematology (Volume 9): Immune Cytopenias. Ed., R. McMillan. Churchill-Livingston, New York, pp 49-67.

Pidard D, TJ Kunicki, J-P Rosa and AT Nurden. 1980. "Human platelet membrane glycoproteins IIb and IIIa may form Ca2+-dependent complexes." In: Protides of the Biological Fluids. 29th Colloquium. Ed., H. Peeters, Pergamon Press, Oxford, pp. 85-88.

Nurden AT, D Dupuis, D Pidard, TJ Kunicki and JP Caen. 1980. "Platelet glycoproteins in relation to human platelet function." In: Platelets: Cellular response mechanisms and their biological significance. Eds. A. Rotman, FA Meyer, C Gitler and A Silberg. John Wiley and Sons, Ltd., London, pp. 119-130.


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